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Citation Information
Biochimie. 2019 Feb;157:111-122. doi: 10.1016/j.biochi.2018.11.007. Epub 2018 Nov 13.
Study of the metabolomic relationship between lung cancer and chronic obstructive pulmonary disease based on direct infusion mass spectrometry.
Callejón-Leblic B, Pereira-Vega A, Vázquez-Gandullo E, Sánchez-Ramos JL, Gómez-Ariza JL, García-Barrera T.
Analytical methods | |||
|---|---|---|---|
| Comparative study | #1 | #2 | |
| Chromatography | DI | DI | |
| Ion source | ESI | ESI | |
| Positive/Negative mode | negative | positive | |
| Mass analyzer | QTOF | QTOF | |
| Identification level | MS/MS | MS/MS | |
Sample information | |||
|---|---|---|---|
| Comparative study | #1 | #2 | |
| Country | Spain | Spain | |
| Specimen | blood | blood | |
| Marker function | diagnosis | diagnosis | |
| Participants(Case) | Cancer type | NSCLC, SCLC | NSCLC, SCLC |
| Stage | II, III, IV | II, III, IV | |
| Number | 30 | 30 | |
| Gender (M,F) | 25, 5 | 25, 5 | |
| Mean age (range) (M,F) | 67 ± 12 | 67 ± 12 | |
| Smoking status | former, current, non-smoker | former, current, non-smoker | |
| Participants(Control) | Type | healthy | healthy |
| Number | 30.0 | 30.0 | |
| Gender (M,F) | 14, 16 | 14, 16 | |
| Mean age (range) (M,F) | 56 ± 14 | 56 ± 14 | |
| Smoking status | former, non-smoker | former, non-smoker | |
Data processing and metabolite identification | |
|---|---|
| Data processing software | – |
| Database search | HMDB, Metlin |
Statistics and concentration information | |
|---|---|
| Differential analysis method | PCA, PLS-DA, one-way ANOVA |
| Classification method | ROC curve |
| Survival analysis method | – |
Metabolites identified in the paper
| Metabolite | Comparative study | Author-emphasized biomarkers | Mean concentration (case) | Mean concentration (control) | Fold change (case/control) | P-value | FDR | VIP |
|---|---|---|---|---|---|---|---|---|
| butyryl-carnitine | – | – | – | – | 2.28 | 0.044 | – | 1.26 |
| pentanoyl-carrnitine | – | – | – | – | 1.81 | 0.032 | – | 2.3 |
| docohexaenoic acid | – | – | – | – | 0.72 | 0.03 | – | 1.43 |
| adenosine | – | – | – | – | 2.86 | 0.026 | – | 1.81 |
| xanthine | – | – | – | – | 1.86 | 0.022 | – | 1.83 |
| LPC(18:0) | – | – | – | – | 1.38 | 0.019 | – | 1.14 |
| phenylalanine | – | – | – | – | 0.56 | 0.015 | – | 2.07 |
| DAG(18:3/18:1) | – | – | – | – | 1.32 | 0.014 | – | 1.12 |
| docopentaenoic acid | – | – | – | – | 1.32 | 0.014 | – | 1.44 |
| PC(16:0/18:0) | – | – | – | – | 1.67 | 0.014 | – | 1.17 |
| PC(18:1/20:3) | – | – | – | – | 3.16 | 0.013 | – | 1.73 |
| oleic acid | – | – | – | – | 1.68 | 0.012 | – | 1.48 |
| stearic acid | – | – | – | – | 1.57 | 0.011 | – | 1.14 |
| adenine | – | – | – | – | 1.1 | 0.005 | – | 1.35 |
| aspartic acid | – | – | – | – | 1.32 | 0.004 | – | 1.02 |
| docosatetraenoic acid | – | – | – | – | 1.34 | 0.004 | – | 1.68 |
| acetic acid | – | – | – | – | 1.36 | 0.003 | – | 1.27 |
| phosphoethanolamine | – | – | – | – | 0.77 | 0.0021 | – | 1.01 |
| TAG(16:0/18:1/18:1) | – | – | – | – | 1.43 | 0.0017 | – | 1.12 |
| palmitoleic acid | – | – | – | – | 1.97 | 0.0016 | – | 1.39 |
| propionylcarnitine | – | – | – | – | 2.55 | 0.0015 | – | 1.51 |
| glutathione | – | – | – | – | 0.48 | 0.0014 | – | 1.64 |
| TAG(16:0/18:2/22:6) | – | – | – | – | 1.69 | 0.0014 | – | 1.3 |
| TAG(14:0/16:0/18:1) | – | – | – | – | 1.62 | 0.0013 | – | 1.16 |
| tyrosine | – | – | – | – | 0.66 | 0.0013 | – | 1.55 |
| creatine | – | – | – | – | 1.33 | 0.0012 | – | 1.89 |
| TAG(18:3/18:2/18:2) | – | – | – | – | 1.4 | 0.0011 | – | 1.07 |
| PC(18:0/22:6) | – | – | – | – | 2.99 | 0.001 | – | 1.67 |
| TAG(18:2/18:2/18:1) | – | – | – | – | 1.66 | 0.001 | – | 1.22 |
| choline | – | – | – | – | 1.49 | 0.0006 | – | 1.2 |
| glucose | – | – | – | – | 1.62 | 0.0005 | – | 1.73 |
| LPC(16:0) | – | – | – | – | 1.66 | 0.0005 | – | 1.01 |
| TAG(18:2/18:2/18:2) | – | – | – | – | 1.51 | 0.0005 | – | 1.38 |
| PC(16:0/18:3) | – | – | – | – | 1.43 | 0.0004 | – | 1.1 |
| PC(16:0/16:0) | – | – | – | – | 1.43 | 0.0003 | – | 1.25 |
| TAG(16:0/16:0/16:0) | – | – | – | – | 1.45 | 0.0003 | – | 1.1 |
| carnitine | – | – | – | – | 1.44 | 0.0002 | – | 2.11 |
| glutamate | – | – | – | – | 1.35 | 0.0002 | – | 1.35 |
| PC(16:0/16:1) | – | – | – | – | 1.43 | 0.0002 | – | 1.4 |
| PC(16:0/20:5) | – | – | – | – | 1.5 | 0.0002 | – | 1.01 |
| PC(18:2/18:2) | – | – | – | – | 1.52 | 0.0002 | – | 1.11 |
| inosine | – | – | – | – | 3.14 | 0.0001 | – | 2.89 |
| phosphocholine | – | – | – | – | 1.4 | 0.0001 | – | 1.81 |
| TAG(16:0/16:0/18:1) | – | – | – | – | 1.43 | 0.0001 | – | 1.39 |
| TAG(16:0/16:0/18:2) | – | – | – | – | 1.67 | 0.0001 | – | 1.21 |
| TAG(16:0/18:2/18:2) | – | – | – | – | 1.35 | 0.0001 | – | 1.01 |
| threonine | – | – | – | – | 1.43 | 0.0001 | – | 2.15 |
| Metabolite | Comparative study | Author-emphasized biomarkers | Cutoff value | AUROC (95%CI) | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|---|
| butyryl-carnitine | – | – | – | 0.66 | – | – | – |
| pentanoyl-carrnitine | – | – | – | 0.61 | – | – | – |
| docohexaenoic acid | – | – | – | 0.65 | – | – | – |
| adenosine | – | – | – | 0.65 | – | – | – |
| xanthine | – | – | – | 0.52 | – | – | – |
| LPC(18:0) | – | – | – | 0.64 | – | – | – |
| phenylalanine | – | – | – | 0.65 | – | – | – |
| DAG(18:3/18:1) | – | – | – | 0.65 | – | – | – |
| docopentaenoic acid | – | – | – | 0.53 | – | – | – |
| PC(16:0/18:0) | – | – | – | 0.67 | – | – | – |
| PC(18:1/20:3) | – | – | – | 0.51 | – | – | – |
| oleic acid | – | – | – | 0.64 | – | – | – |
| stearic acid | – | – | – | 0.55 | – | – | – |
| adenine | – | – | – | 0.58 | – | – | – |
| aspartic acid | – | – | – | 0.53 | – | – | – |
| docosatetraenoic acid | – | – | – | 0.55 | – | – | – |
| acetic acid | – | – | – | 0.54 | – | – | – |
| phosphoethanolamine | – | – | – | 0.74 | – | – | – |
| TAG(16:0/18:1/18:1) | – | – | – | 0.67 | – | – | – |
| palmitoleic acid | – | – | – | 0.62 | – | – | – |
| propionylcarnitine | – | – | – | 0.57 | – | – | – |
| glutathione | – | – | – | 0.6 | – | – | – |
| TAG(16:0/18:2/22:6) | – | – | – | 0.61 | – | – | – |
| TAG(14:0/16:0/18:1) | – | – | – | 0.63 | – | – | – |
| tyrosine | – | – | – | 0.73 | – | – | – |
| creatine | – | – | – | 0.7 | – | – | – |
| TAG(18:3/18:2/18:2) | – | – | – | 0.69 | – | – | – |
| PC(18:0/22:6) | – | – | – | 0.61 | – | – | – |
| TAG(18:2/18:2/18:1) | – | – | – | 0.55 | – | – | – |
| choline | – | – | – | 0.66 | – | – | – |
| glucose | – | – | – | 0.65 | – | – | – |
| LPC(16:0) | – | – | – | 0.72 | – | – | – |
| TAG(18:2/18:2/18:2) | – | – | – | 0.72 | – | – | – |
| PC(16:0/18:3) | – | – | – | 0.65 | – | – | – |
| PC(16:0/16:0) | – | – | – | 0.68 | – | – | – |
| TAG(16:0/16:0/16:0) | – | – | – | 0.64 | – | – | – |
| carnitine | – | – | – | 0.73 | – | – | – |
| glutamate | – | – | – | 0.71 | – | – | – |
| PC(16:0/16:1) | – | – | – | 0.74 | – | – | – |
| PC(16:0/20:5) | – | – | – | 0.72 | – | – | – |
| PC(18:2/18:2) | – | – | – | 0.62 | – | – | – |
| inosine | – | – | – | 0.69 | – | – | – |
| phosphocholine | – | – | – | 0.71 | – | – | – |
| TAG(16:0/16:0/18:1) | – | – | – | 0.72 | – | – | – |
| TAG(16:0/16:0/18:2) | – | – | – | 0.66 | – | – | – |
| TAG(16:0/18:2/18:2) | – | – | – | 0.6 | – | – | – |
| threonine | – | – | – | 0.76 | – | – | – |
Paper graphical summary
