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Citation Information
BMC Cancer. 2010 Dec 22;10:690. doi: 10.1186/1471-2407-10-690.
Possibility of multivariate function composed of plasma amino acid profiles as a novel screening index for non-small cell lung cancer: a case control study.
Maeda J, Higashiyama M, Imaizumi A, Nakayama T, Yamamoto H, Daimon T, Yamakado M, Imamura F, Kodama K.
Analytical methods | ||
|---|---|---|
| Comparative study | #1 | |
| Chromatography | LC | |
| Ion source | ESI | |
| Positive/Negative mode | positive | |
| Mass analyzer | quadrupole | |
| Identification level | – | |
Sample information | ||
|---|---|---|
| Comparative study | #1 | |
| Country | Japan | |
| Specimen | plasma | |
| Marker function | – | |
| Participants(Case) | Cancer type | NSCLC |
| Stage | I, II, III, IV | |
| Number | 141 | |
| Gender (M,F) | 93, 48 | |
| Mean age (range) (M,F) | 62.7 ± 9.2 | |
| Smoking status | former, current, non-smoker | |
| Participants(Control) | Type | healthy |
| Number | 423.0 | |
| Gender (M,F) | 279, 144 | |
| Mean age (range) (M,F) | 61.1 ± 8.7 | |
| Smoking status | former, current, non-smoker | |
Data processing and metabolite identification | |
|---|---|
| Data processing software | Xcalibur |
| Database search | – |
Statistics and concentration information | |
|---|---|
| Differential analysis method | Mann-Whitney U-test, PCA |
| Classification method | ROC curve |
| Survival analysis method | – |
Metabolites identified in the paper
| Metabolite | Comparative study | Author-emphasized biomarkers | Mean concentration (case) | Mean concentration (control) | Fold change (case/control) | P-value | FDR | VIP |
|---|---|---|---|---|---|---|---|---|
| Ile | – | V | 84.3 ± 22.1 μM | 69.7±17.5 μM | – | <0.001 | – | – |
| Orn | – | V | 67.6 ± 19.7 μM | 54.4±12.3 μM | – | <0.001 | – | – |
| Pro | – | V | 168 ± 43.6 μM | 150.9±41.4 μM | – | <0.001 | – | – |
| thr | – | V | 115.6 ± 28.6 μM | 115.9 ± 25.4 μM | – | 0.92 | – | – |
| Asn | – | V | 45.1 ± 8.2 μM | 44.8±7.2 μM | – | 0.72 | – | – |
| trp | – | V | 59.3 ± 12 μM | 59.8±10.9 μM | – | 0.67 | – | – |
| Glu | – | V | 46.7 ± 19.4 μM | 45.7±19.5 μM | – | 0.6 | – | – |
| Gln | – | V | 580.5 ± 93.3 μM | 587.9±83.5 μM | – | 0.4 | – | – |
| val | – | V | 244.8 ± 47.5 μM | 239.8±46.1 μM | – | 0.28 | – | – |
| ABA | – | V | 24.2 ± 8.4 μM | 23.2±6.9 μM | – | 0.2 | – | – |
| Cit | – | V | 34.4 ± 10.6 μM | 33.2±8.3 μM | – | 0.2 | – | – |
| Arg | – | V | 101.3 ± 21.6 μM | 98.1±17.8 μM | – | 0.12 | – | – |
| met | – | V | 29.4 ± 6.1 μM | 28±5.2 μM | – | 0.013 | – | – |
| His | – | V | 77.3 ± 15 μM | 80.8±10.7 μM | – | 0.01 | – | – |
| Leu | – | V | 131.8 ± 34.3 μM | 122.4±27.6 μM | – | 0.003 | – | – |
| Ser | – | V | 117.1 ± 19.9 μM | 111.4±17.9 μM | – | 0.003 | – | – |
| Lys | – | V | 211.5 ± 36.2 μM | 200.3±34.1 μM | – | 0.001 | – | – |
| Phe | – | V | 67.9 ± 12.2 μM | 63.9±11.2 μM | – | 0.001 | – | – |
| tyr | – | V | 80.7 ± 15.6 μM | 75.8±15.5 μM | – | 0.001 | – | – |
| Ala | – | V | 422.3 ± 97.4 μM | 383.7±88.9 μM | – | 0 | – | – |
| Gly | – | V | 263.6 ± 63.2 μM | 237±57.3 μM | – | 0 | – | – |
| Metabolite | Comparative study | Author-emphasized biomarkers | Cutoff value | AUROC (95%CI) | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|---|
| Ile | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Orn | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Pro | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| thr | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Asn | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| trp | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Glu | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Gln | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| val | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| ABA | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Cit | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Arg | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| met | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| His | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Leu | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Ser | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Lys | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Phe | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| tyr | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Ala | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
| Gly | – | V | – | combination of 21 amino acid: 0.812 | – | – | – |
Paper graphical summary
