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Citation Information
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Sep 1;966:147-53. doi: 10.1016/j.jchromb.2014.04.047. Epub 2014 May 2.
Serum metabolic profiling study of lung cancer using ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry.
Li Y, Song X, Zhao X, Zou L, Xu G.
Analytical methods | ||||
|---|---|---|---|---|
| Comparative study | #1 | #2 | #3 | |
| Chromatography | LC | LC | LC | |
| Ion source | – | – | – | |
| Positive/Negative mode | positive | negative | both | |
| Mass analyzer | Q-TOF | Q-TOF | Q-TOF | |
| Identification level | MS/MS | MS/MS | MS/MS | |
Sample information | ||||
|---|---|---|---|---|
| Comparative study | #1 | #2 | #3 | |
| Country | China | China | China | |
| Specimen | serum | serum | serum | |
| Marker function | diagnosis | diagnosis | diagnosis | |
| Participants(Case) | Cancer type | SCLC, NSCLC | SCLC, NSCLC | SCLC, NSCLC |
| Stage | – | – | – | |
| Number | 23 | 23 | 23 | |
| Gender (M,F) | 12, 11 | 12, 11 | 12, 11 | |
| Mean age (range) (M,F) | 63.0 ± 9.8 / 59.4 ± 5.8 | 63.0 ± 9.8 / 59.4 ± 5.8 | 63.0 ± 9.8 / 59.4 ± 5.8 | |
| Smoking status | – | – | – | |
| Participants(Control) | Type | healthy | healthy | healthy |
| Number | 23 | 23 | 23 | |
| Gender (M,F) | 11, 12 | 11, 12 | 11, 12 | |
| Mean age (range) (M,F) | 51.0 ± 11.1 / 56.3 ± 14.3 | 51.0 ± 11.1 / 56.3 ± 14.3 | 51.0 ± 11.1 / 56.3 ± 14.3 | |
| Smoking status | – | – | – | |
Data processing and metabolite identification | |
|---|---|
| Data processing software | MarkerLynx |
| Database search | METLIN, HMDB, KEGG |
Statistical analysis | |
|---|---|
| Differential analysis method | PCA, PLS-DA, OSC-PLS-DA, student’s t-test |
| Classification method | ROC curve analysis |
| Survival analysis method | – |
Lung cancer-related metabolites identified in the paper
| Metabolite | Author-emphasized biomarkers | Mean concentration (case) | Mean concentration (control) | Fold change (case/control) | P-value | FDR | VIP |
|---|---|---|---|---|---|---|---|
| 1-arachidonoyl glycerol | – | – | – | – | 0.05 | – | 1.50 |
| arachidonic acid | – | – | – | – | 0.05 | – | 1.50 |
| carnitine 10:1 | – | – | – | – | 0.05 | – | 1.40 |
| carnitine 8:1 | – | – | – | – | 0.05 | – | 1.10 |
| choline | V | – | – | – | 0.05 | – | 1.20 |
| DG (38:8) | – | – | – | – | 0.05 | – | 3.20 |
| fatty acid amide C20:0 | – | – | – | – | 0.05 | – | 1.70 |
| fatty acid amide C20:1 | – | – | – | – | 0.05 | – | 3.90 |
| fatty acid amide C22:1 | – | – | – | – | 0.05 | – | 1.30 |
| fatty acid amide C22:2 | – | – | – | – | 0.05 | – | 1.30 |
| fatty acid amide C24:1 | – | – | – | – | 0.05 | – | 8.70 |
| linoleic acid | V | – | – | – | 0.05 | – | 6.30 |
| LPC(16:0) | – | – | – | – | 0.05 | – | 8.60 |
| LPC 16:0 isomer | – | – | – | – | 0.05 | – | 3.70 |
| LPC(18:0) | – | – | – | – | 0.05 | – | 6.30 |
| LPC(18:2) | – | – | – | – | 0.05 | – | 6.40 |
| LPC(20:4) | – | – | – | – | 0.05 | – | 2.40 |
| LPC(20:5) | – | – | – | – | 0.05 | – | 2.30 |
| LPC(22:3) | – | – | – | – | 0.05 | – | 1.40 |
| LPC C16 1 | – | – | – | – | 0.05 | – | 1.30 |
| LPC C20 3 | – | – | – | – | 0.05 | – | 1.10 |
| oleic acid | – | – | – | – | 0.05 | – | 7.40 |
| palmitic acid | – | – | – | – | 0.05 | – | 6.00 |
| palmitoleic acid | – | – | – | – | 0.05 | – | 1.50 |
| SM(d18:1/14:0) | – | – | – | – | 0.05 | – | 1.60 |
| stearic acid | – | – | – | – | 0.05 | – | 2.60 |
| Metabolite | Author-emphasized biomarkers | Cutoff value | AUROC (95%CI) | Sensitivity (%) | Specificity (%) | Accuracy (%) |
|---|---|---|---|---|---|---|
| 1-arachidonoyl glycerol | – | – | – | – | – | – |
| arachidonic acid | – | – | – | – | – | – |
| carnitine 10:1 | – | – | – | – | – | – |
| carnitine 8:1 | – | – | – | – | – | – |
| choline | V | – | choline+linoleic acid=1 | 1 | 0.96 | – |
| DG (38:8) | – | – | – | – | – | – |
| fatty acid amide C20:0 | – | – | – | – | – | – |
| fatty acid amide C20:1 | – | – | – | – | – | – |
| fatty acid amide C22:1 | – | – | – | – | – | – |
| fatty acid amide C22:2 | – | – | – | – | – | – |
| fatty acid amide C24:1 | – | – | – | – | – | – |
| linoleic acid | V | – | choline+linoleic acid=1 | 1 | 0.96 | – |
| LPC(16:0) | – | – | – | – | – | – |
| LPC 16:0 isomer | – | – | – | – | – | – |
| LPC(18:0) | – | – | – | – | – | – |
| LPC(18:2) | – | – | – | – | – | – |
| LPC(20:4) | – | – | – | – | – | – |
| LPC(20:5) | – | – | – | – | – | – |
| LPC(22:3) | – | – | – | – | – | – |
| LPC C16 1 | – | – | – | – | – | – |
| LPC C20 3 | – | – | – | – | – | – |
| oleic acid | – | – | – | – | – | – |
| palmitic acid | – | – | – | – | – | – |
| palmitoleic acid | – | – | – | – | – | – |
| SM(d18:1/14:0) | – | – | – | – | – | – |
| stearic acid | – | – | – | – | – | – |
Paper graphical summary
